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Promotions

Multiple Promotions Current until Dec 31 2008!!

We are currently running a number of promtions that will allow all clients to save $$$ on custom hybridoma development with ImmunoPrecise.


Also, if you submit ANY quote request you will automatically be entered into our draw for a complete custom hybridoma project, absolutely free of charge. The draw closes Jan 31 so enter today. One entry per customer please.

Accolades

Customer Feedback

Listen to what IPA's existing & past customers have to say about our products & custom antibody development services.

Accreditation

National Institutes of Health

IPA is a compliant member by the Office of Laboratory Animal Welfare (OLAW) of the the Department of Health and Human Services (HHS), USA and has been awarded with a 5-year Animal Welfare Assurance Number.

Canadian Council on Animal Care

IPA is a member of the the Canadian Council on Animal Care. The CCAC provides certification and policies on ethical animal care and use.

Better Business Bureau

IPA is a member of the Better Business Bureau. The BBB is a private, non-profit organization, dedicated to promoting fairness and honesty in the marketplace.

Citations

Peer Reviewed Publications

Review the expanding list of publications sourcing IPA's catalog products & exclusively developed custom mAb hybridomas.

Quick Links

Quote Request Form

To expedite receiving a quote from IPA for your custom antibody development project, fill in the on-line submission form or download the following form and email or fax to (250) 483-0309. Quotes issued in less than 24 hrs.

Product Order Form

To order any of IPA's antibody products, download the following form and email or fax to (250) 483-0309. Confirmation in less than 24 hrs.

Antigen Infomation Sheet

To initiate the immunization phase of your custom antibody development project, download the following form and submit it with the shipment of your antigen.


Custom Monoclonal Antibody Development

IPA advantage... Time-saving single-step cloning
Certified facility... Quality service guaranteed
Professional experience... Highest success rate


Custom Monoclonal Antibody Development - Rapid Prime


  • IPA Rapid Prime - Produce mAbs in 65 days *.
  • Certified facility - Quality service guaranteed.
  • Professional experience - Over 95% success rate.

Service

IPA’s custom monoclonal antibody development service is fast and efficient. IPA’s trademark Rapid Prime Method, IPA can custom develop monoclonal antibodies to an array of antigens including purified proteins, recombinant proteins and small molecules (bacteria, viruses) in 65 days. We also offer a standard immunization procedure which is still accelerated by our patented single-step cloning method which also saves time compared to our competitors.


Rapid Prime - Custom Monoclonal Antibody Development

Phase of The Project

Time & Costing

I. Immunization
  • Immunization of 4 Balb/c mice.

Antigen requirements

  • Recombinant or Purified Molecules
    • 3mg of Antigen
  • Soluble Antibens
    • Antigens in solution must be prepare in a PBS buffered solution, pH 7.4 at a concentration of 0.5 - 2 mg/mL

17 Days

II. Hybridoma Fusion
  • Lymphocytes from all mice are pooled and purified.
  • Lymphocytes are fused with SP2/0 myelomas.
  • Fused cells are cloned on Clone-EZ semi-solid media.
  • Hybridoma clones are selected and grown in individual wells.

14 days

III. Screening of Hybridoma Clones
  • ELISA evaluation of tissue culture supernatants using screening antigen. (Takes Place day 65)
  • Positive clones screened with a control antigen to select against non-specific clones.
  • Positive clones cultured to select for stable expressing clones.

27 days

IV. Cryopreservation
  • Five (5) vials, of up to 5 positive clones, will be sent to the Client by courier on dry ice and 3 vials per cell line will be stored with IPA as an emergency stock. The time period to cryopreserve the clones is 2 weeks.
  • Up to 5 positive clones, 3 vials per clone, will be cryopreserved for a period of 1 month on-site at IPA.

7 days

 

Approx 68 days or 9-10 weeks total
$7900

Optional V. Ascites Production or Antibody Concentration

As an option for the Client, clones can be injected into mice for ascites production in mice. As a mouse friendly alternative, the clones can be concentrated in Integra Flasks. The time period to concentrate the antibody is 4-5 weeks.

6 weeks

Confidentiality

IPA maintains all information under the strictest confidentiality. All information and/or materials supplied by the client will be used as directed. Upon completion of the project, all materials will be either returned or disposed of as directed by the client. IPA is willing to execute confidentiality agreements with its clients.


For more information about our methodology, click here.